pBad-Xis/Int Reset Flipper
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||38207||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 2800
Vector typeSynthetic Biology ; BioBrick cloning vector
Growth in Bacteria
- Promoter pBAD
Cloning Information for Gene/Insert 1
- Cloning method Unknown
- 5′ sequencing primer pBAD-F (Common Sequencing Primers)
Gene/Insert nameBxb1 Integrase
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
In the reset circuit, PBAD controls expression of a polycistron encoding excisionase with a strong RBS followed by Bxb1 integrase with a GTG start codon to decrease its translation rate
and increase the excisionase to integrase ratio. This construct was cloned in a plasmid
with a regulated ColE1 origin of replication (J64100, 50-70 copies, chloramphenicol resistance).
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pBad-Xis/Int Reset Flipper was a gift from Drew Endy (Addgene plasmid # 38207 ; http://n2t.net/addgene:38207 ; RRID:Addgene_38207)
For your References section:Rewritable digital data storage in live cells via engineered control of recombination directionality. Bonnet J, Subsoontorn P, Endy D. Proc Natl Acad Sci U S A. 2012 Jun 5;109(23):8884-9. Epub 2012 May 21. 10.1073/pnas.1202344109 PubMed 22615351