Addgene - pBad-Int Set Flipper Plasmid Data

Plasmid 38209: pBad-Int Set Flipper

Gene/insert name:
Bxb1 Integrase
Fusion protein or tag:
His
Terminal:
N terminal on insert
Vector backbone:
pSB3K1
(Search Vector Database)
Vector type:
Synthetic ; BioBrick cloning vector
Promoter:
pBAD/AraC
Cloning method:
Unknown
5' sequencing primer:
pBAD-F List of Sequencing Primers
Bacterial resistance(s)
Kanamycin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
Unknown
Sequence: View sequences (3)
Map:
View map
Principal Investigator:
Drew Endy
Terms and Licenses:
MTA

Comments:

The Bxb1 integrase was cloned downstream of the PBAD/AraC promoter (BBa_I0500) on pSB3K1 plasmid bearing a p15A origin of replication (15-20 copies). This version of the integrase has a 6-His-tag which we found to stabilize the protein. Therefore, a weak RBS (BBa_B0031) and a LAA ssrA tag was added to reduce the basal expression of the enzyme.

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Rewritable digital data storage in live cells via engineered control of recombination directionality. Bonnet et al (Proc Natl Acad Sci U S A. 2012 Jun 5;109(23):8884-9. Epub 2012 May 21. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 38209" in your Materials and Methods section.