pLenti-puro
(Plasmid #39481)

• Purpose: (Empty Backbone) Tetracycline inducible 3rd generation lentiviral vector
• Depositing Lab: Ie-Ming Shih
• Publication: Guan et al Cancer Res. 2011 Nov 1;71(21):6718-27. Epub 2011 Sep 7.

Backbone

• Vector backbone: pLenti4/TO/V5-DEST
• Backbone manufacturer: Invitrogen
• Modifications to backbone: Removed the Gateway elements between the 2 EcoRV sites and replaced the Zeocin resistance gene with a puromycin resistance gene
• Vector type: Mammalian Expression, Lentiviral
• Promoter: CMV
• Selectable markers: Puromycin
• Tag / Fusion Protein:
  • V5 and 6XHis (C terminal on backbone)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): Stbl3
• Growth instructions: See Depositor Comments below.
• Copy number: High Copy

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ sequencing primer: CMV-fwd
• 3′ sequencing primer: V5-rev and BGH-Rev

Resource Information

• Addgene Notes:
  • Addgene diagnostic digest
  • Addgene Diagnostic Digest
• Articles Citing this Plasmid:
  • 71 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please note this plasmid appears to be unstable during growth in bacteria. We recommend screening 3-5 smaller colonies after streaking from the original stab. It may be necessary to cut the plasmid with a single-cutting enzyme, gel purify the high molecular weight band, and religate to restore the plasmid.

Information for Cloning Grade DNA (Catalog # 39481-DNA.cg) ( Back to top )

Purpose

Cloning grade DNA is suitable for use in PCR, cloning reactions, or transformation into E. coli. The purity and amount is not suitable for direct transfections.

Delivery

• Amount: 2 µg
• Guaranteed Concentration: 100 ng/µl +/- 5 ng/µl
• Pricing: $105 USD
• Storage: DNA can be stored at 4℃ (short term) or -20℃ (long term).

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Quality Control

Addgene has verified this plasmid using Next Generation Sequencing. Results are available here