Addgene - pEGFP-Akt-DD Plasmid Data

Plasmid 39536: pEGFP-Akt-DD

Gene/insert name:
Akt(K179M)
Insert size:
1600
Species:
M. musculus (mouse)
Entrez Gene:
Akt1 (Akt, PKB, PKB/Akt, PKBalpha, Rac)
Fusion protein or tag:
6G linker
Terminal:
N terminal on insert
Fusion protein or tag:
EGFP
Terminal:
N terminal on backbone
Mutation:
DD = T308D and S473D
Vector backbone:
pEGFP-C1
(Search Vector Database)
Backbone manufacturer:
Clontech
Vector type:
Mammalian Expression
Backbone size w/o insert (bp):
4700
Cloning site 5':
SalI
Site destroyed during cloning:
No
Cloning site 3':
BamHI
Site destroyed during cloning:
No
5' sequencing primer:
EGFP-C List of Sequencing Primers
3' sequencing primer:
sv40pA-R
Bacterial resistance(s)
Kanamycin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
Unknown
Sequence: View sequences (2)
Principal Investigator:
Julian Downward
Terms and Licenses:
MTA

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Akt/PKB localisation and 3' phosphoinositide generation at sites of epithelial cell-matrix and cell-cell interaction. Watton et al (Curr Biol. 1999 Apr 22;9(8):433-6. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 39536" in your Materials and Methods section.