|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||43805||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 5597
- Total vector size (bp) 6110
Vector typeMammalian Expression, Luciferase
Growth in Bacteria
Copy numberHigh Copy
Gene/Insert nameHes1 Promoter (-467 to +46) RBPj(-)
Alt namehairy and enhancer of split 1 Promoter (-467 to +46) RBPj(-)
Alt namehairy and enhancer of split 1
SpeciesM. musculus (mouse)
Insert Size (bp)513
MutationContains the murine Hes1 promoter (-467 to +46), lacks the RBP-J site (see below)
Entrez GeneHes1 (a.k.a. Hr, Hry, bHLHb3, bHLHb39)
- Promoter Hes1 promoter fragment (-467 to +46) (RBPj-)
/ Fusion Protein
- Firefly luciferase (C terminal on backbone)
- Cloning method Unknown
- 5′ sequencing primer F1ori-F (GTGGACTCTTGTTCCAAACTGG)
- 3′ sequencing primer LucNrev (Common Sequencing Primers)
Alternate plasmid name: Hes1/RBP-J (-)
This construct contains the murine Hes1 promoter lacking the RBP-J site.
Relevant sequence portion:
WT: -86 /TGTGGGAAAGAAAGTTTGGGAAGTTT/ -61
Rbp J(-): -86 /TGTG[CTGC]AGAAAgtTT[CTCG]AGTTT/ -61 (mutated sequences are bracketed)
In Rbp J(-), PstI (CTGCAG) and XhoI (CTCGAG) sites are introduced.
Addgene quality control discovered a 2-base deletion (lower case letters) in the murine Hes1 promoter. This should not affect the function, and the vector is OK.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pHes1(467 RBPj(-))-luc was a gift from Ryoichiro Kageyama (Addgene plasmid # 43805 ; http://n2t.net/addgene:43805 ; RRID:Addgene_43805)
For your References section:Structure, chromosomal locus, and promoter of mouse Hes2 gene, a homologue of Drosophila hairy and Enhancer of split. Nishimura M, Isaka F, Ishibashi M, Tomita K, Tsuda H, Nakanishi S, Kageyama R. Genomics. 1998 Apr 1;49(1):69-75. 10.1006/geno.1998.5213 PubMed 9570950
Map uploaded by the depositor.