JDS246
(Plasmid #43861)

• Purpose: Expresses mammalian codon optimized Cas9 nuclease with C-term 3X FLAG from CMV and T7 promoters
• Depositing Lab: Keith Joung
• Publication: Joung lab unpublished CRISPR plasmids (unpublished)

Backbone

• Vector backbone: unknown
• Vector type: Mammalian Expression, CRISPR

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): XL1 Blue
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: mammalian codon-optimized streptococcus pyogenes Cas9 - 3X Flag
• Alt name: Cas9-003
• Species: streptococcus pyogenes
• Promoter: CMV
• Tag / Fusion Protein:
  • 3X FLAG (C terminal on insert)

Cloning Information

• Cloning method: Unknown
• 5′ sequencing primer: CMV-F
• 3′ sequencing primer: OK569 (5’-TAGAAGGCACAGTCGAGG-3’)

Resource Information

• Addgene Notes:
  • Addgene Diagnostic Digest
• Articles Citing this Plasmid:
  • 48 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Mammalian codon-optimized Cas9 expression vector for use with MLM3636 (Addgene plasmid# 43860--human cell gRNA expression vector)

Plasmid Features:
CAG enhancer-CMV promoter = 7466-370
Cas9 = 409-4512
NLS = 4519-4539
3X FLAG = 4546-4611
BGHpA = 4612-4839

For more information on Joung Lab CRISPR Plasmids please refer to: http://www.addgene.org/crispr/jounglab/