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SP6-hCas9-Ce-mRNA
(Plasmid #47911)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 47911 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    Bluescript
  • Backbone manufacturer
    Stratagene
  • Backbone size w/o insert (bp) 3000
  • Total vector size (bp) 7115
  • Vector type
    CRISPR

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    hCas9 with C. elegans 5' and 3' UTRs
  • Species
    H. sapiens (human), C. elegans (nematode)
  • Promoter SP6

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRV (destroyed during cloning)
  • 3′ cloning site KpnI (not destroyed)
  • 5′ sequencing primer T3
  • 3′ sequencing primer T7
  • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Contains an insert modified from Addgene plasmid #41815 hCas9
  • Terms and Licenses
  • Industry Terms
    • Not Available to Industry
  • Article Citing this Plasmid
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    SP6-hCas9-Ce-mRNA was a gift from Paul Sternberg (Addgene plasmid # 47911 ; http://n2t.net/addgene:47911 ; RRID:Addgene_47911)
  • For your References section:

    Transgene-Free Genome Editing in Caenorhabditis elegans Using CRISPR-Cas. Chiu H, Schwartz HT, Antoshechkin I, Sternberg PW. Genetics. 2013 Aug 26. 10.1534/genetics.113.155879 PubMed 23979577