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pHAGE NFkB-TA-LUC-UBC-GFP-W
(Plasmid #49343)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 49343 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pHAGE
  • Backbone manufacturer
    Richard Mulligan
  • Backbone size w/o insert (bp) 4874
  • Total vector size (bp) 8772
  • Vector type
    Lentiviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert 1

  • Gene/Insert name
    eGFP
  • Insert Size (bp)
    720
  • Entrez Gene
    eGFP (a.k.a. pPRS3a_01)

Gene/Insert 2

  • Gene/Insert name
    Luciferase
  • Insert Size (bp)
    1653

Gene/Insert 3

  • Gene/Insert name
    NFkB consensus binding sequence
  • Insert Size (bp)
    66

Gene/Insert 4

  • Gene/Insert name
    TA
  • Alt name
    minimal TA promoter
  • Insert Size (bp)
    7

Gene/Insert 5

  • Gene/Insert name
    UBC
  • Alt name
    ubiquitin-C promoter
  • Insert Size (bp)
    411

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pHAGE NFkB-TA-LUC-UBC-GFP-W was a gift from Darrell Kotton (Addgene plasmid # 49343 ; http://n2t.net/addgene:49343 ; RRID:Addgene_49343)
  • For your References section:

    Lentiviral delivery of RNAi for in vivo lineage-specific modulation of gene expression in mouse lung macrophages. Wilson AA, Kwok LW, Porter EL, Payne JG, McElroy GS, Ohle SJ, Greenhill SR, Blahna MT, Yamamoto K, Jean JC, Mizgerd JP, Kotton DN. Mol Ther. 2013 Apr;21(4):825-33. doi: 10.1038/mt.2013.19. Epub 2013 Feb 12. 10.1038/mt.2013.19 PubMed 23403494