pAAV-EF1a-DIO-mCherry
(Plasmid #50462)

• Purpose: Double floxed mCherry under the control of EF1a promoter
• Depositing Lab: Bryan Roth
• Publication: Roth lab DREADDs (unpublished)

Ordering

Item	Catalog #	Description	Quantity	Price (USD)
Plasmid	50462	Standard format: Plasmid sent in bacteria as agar stab	1	$75	Add to Cart

This material is available to academics and nonprofits only.

Backbone

• Vector backbone: pAAV
  (Search Vector Database)
• Backbone size w/o insert (bp): 4818
• Total vector size (bp): 6314
• Vector type: AAV

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin
• Growth Temperature: 37°C
• Growth Strain(s): Stbl3
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: mCherry
• Species: Aequorea victoria
• Insert Size (bp): 715
• Promoter: EF1a
• Tag / Fusion Protein:
  • N/A

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: Asc I (not destroyed)
• 3′ cloning site: Nhe I (not destroyed)
• 5′ sequencing primer: TTTTTGAGTTTGGATCTTGG
• 3′ sequencing primer: GCATTAAAGCAGCGTATCCACATAGC

Resource Information

• Addgene Notes:
  • Addgene diagnostic digest
• Terms and Licenses:
  • UBMTA
  • genOway Notice of RIghts
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry
• Articles Citing this Plasmid:
  • 5 References

Depositor Comments

For more information, see: http://pdspit3.mml.unc.edu/projects/dreadd/wiki/WikiStart