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pOTTC292 - pAAV EF1a floxed hChR2(H134R)-EYFP
(Plasmid #50834)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 50834 Plasmid sent as bacteria in agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    Addgene 20298
  • Backbone manufacturer
    Karl Deisseroth
  • Backbone size w/o insert (bp) 5500
  • Total vector size (bp) 7266
  • Modifications to backbone
    Removed double floxed inverted open reading frame (hChR2-EYFP) using KpnI and EcoRI digestion. Used In-Fusion cloning to recombine backbone with PCR-based insert (KpnI-loxP-hChR2-EYFP-loxP-EcoRI).
  • Vector type
    Mammalian Expression, AAV, Cre/Lox

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    30°C
  • Growth Strain(s)
    Stbl3
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    hChR2 (H134R)
  • Alt name
    Channel Rhodopsin 2
  • Species
    C. reinhardtii
  • Mutation
    H134R
  • GenBank ID
    EF474017
  • Promoter EF1a
  • Tag / Fusion Protein
    • EYFP (C terminal on insert)

Cloning Information

  • Cloning method Ligation Independent Cloning
  • 5′ sequencing primer EF1a_F1
  • 3′ sequencing primer WPRE_R1
  • (Common Sequencing Primers)

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pOTTC292 - pAAV EF1a floxed hChR2(H134R)-EYFP was a gift from Brandon Harvey (Addgene plasmid # 50834)