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pKLV-U6gRNA(BbsI)-PGKpuro2ABFP
(Plasmid #50946)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 50946 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pBluescript
  • Backbone manufacturer
    Agilent
  • Backbone size w/o insert (bp) 8102
  • Total vector size (bp) 8118
  • Vector type
    Mammalian Expression, Lentiviral, CRISPR
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    None
  • Promoter U6

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BbsI (not destroyed)
  • 3′ cloning site BbsI (not destroyed)
  • 5′ sequencing primer U6
  • (Common Sequencing Primers)

Resource Information

Depositor Comments

This is a 3rd generation lentiviral vector and is successfully packaged with ViraPower Lentiviral Expression systems (Invitrogen).

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pKLV-U6gRNA(BbsI)-PGKpuro2ABFP was a gift from Kosuke Yusa (Addgene plasmid # 50946 ; http://n2t.net/addgene:50946 ; RRID:Addgene_50946)
  • For your References section:

    Genome-wide recessive genetic screening in mammalian cells with a lentiviral CRISPR-guide RNA library. Koike-Yusa H, Li Y, Tan EP, Velasco-Herrera Mdel C, Yusa K. Nat Biotechnol. 2014 Mar;32(3):267-73. doi: 10.1038/nbt.2800. Epub 2013 Dec 23. 10.1038/nbt.2800 PubMed 24535568