PurposeAn empty gRNA expression vector
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||50946||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 8102
- Total vector size (bp) 8118
Vector typeMammalian Expression, Lentiviral, CRISPR
Growth in Bacteria
Growth Strain(s)NEB Stable
Copy numberHigh Copy
- Promoter U6
- Cloning method Restriction Enzyme
- 5′ cloning site BbsI (not destroyed)
- 3′ cloning site BbsI (not destroyed)
- 5′ sequencing primer U6 (Common Sequencing Primers)
A portion of this plasmid was derived from a plasmid made byFUW-OSKM was obtained from Addgene which distributes it on behalf of Whitehead Institute for Biomedical Research.
Terms and Licenses
Articles Citing this Plasmid
This is a 3rd generation lentiviral vector and is successfully packaged with ViraPower Lentiviral Expression systems (Invitrogen).
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pKLV-U6gRNA(BbsI)-PGKpuro2ABFP was a gift from Kosuke Yusa (Addgene plasmid # 50946 ; http://n2t.net/addgene:50946 ; RRID:Addgene_50946)
For your References section:Genome-wide recessive genetic screening in mammalian cells with a lentiviral CRISPR-guide RNA library. Koike-Yusa H, Li Y, Tan EP, Velasco-Herrera Mdel C, Yusa K. Nat Biotechnol. 2014 Mar;32(3):267-73. doi: 10.1038/nbt.2800. Epub 2013 Dec 23. 10.1038/nbt.2800 PubMed 24535568