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AAV-flex-ReaChR-citrine
(Plasmid #50955)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 50955 Plasmid sent as bacteria in agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    AAV2
  • Backbone size w/o insert (bp) 4844
  • Total vector size (bp) 6630
  • Modifications to backbone
    two Lox sites are used to flanked the inverted ReaChR-citrine gene.
  • Vector type
    AAV

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Growth instructions
    Regular growing condition.
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    ReaChR-citrine
  • Species
    Synthetic
  • Insert Size (bp)
    1776
  • GenBank ID
    KF448069
  • Promoter human synapsin promoter
  • Tag / Fusion Protein
    • citrine (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NheI (not destroyed)
  • 3′ cloning site AscI (not destroyed)
  • 5′ sequencing primer none
  • 3′ sequencing primer GFP
  • (Common Sequencing Primers)

Resource Information

Depositor Comments

inverted ReaChR-citrine inserted between two lox sites. WPRE is used before SV40 polyA.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    AAV-flex-ReaChR-citrine was a gift from Roger Tsien (Addgene plasmid # 50955)
  • For your References section:

    ReaChR: a red-shifted variant of channelrhodopsin enables deep transcranial optogenetic excitation. Lin JY, Knutsen PM, Muller A, Kleinfeld D, Tsien RY. Nat Neurosci. 2013 Oct;16(10):1499-508. doi: 10.1038/nn.3502. Epub 2013 Sep 1. 10.1038/nn.3502 PubMed 23995068