|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||51004||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 8344
- Total vector size (bp) 9655
Vector typeMammalian Expression, Lentiviral
Selectable markersZeo marker is outside the LTRs and will not be packaged into virus.
Growth in Bacteria
Growth Strain(s)NEB Stable
Copy numberLow Copy
SpeciesH. sapiens (human), Synthetic; Jellyfish
Insert Size (bp)1131
Entrez GeneH2BC11 (a.k.a. H2B/r, H2BFR, H2BJ, HIST1H2BJ)
- Promoter rat synapsin
/ Fusion Protein
- Cloning method Restriction Enzyme
- 5′ cloning site BamH1 (unknown if destroyed)
- 3′ cloning site Not1 (not destroyed)
- 5′ sequencing primer EGFP-N
- 3′ sequencing primer EGFP-C (Common Sequencing Primers)
Terms and Licenses
Article Citing this Plasmid
Please note that the H2B sequence in this plasmid was derived from H2B-GFP (Addgene plasmid# 11680) and contains the same differences (D26G and V119I) compared to GenBank reference sequences, such as NP_066402.2.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pLenti.Syn(0.5).H2B-eGFP.W was a gift from Rusty Lansford (Addgene plasmid # 51004 ; http://n2t.net/addgene:51004 ; RRID:Addgene_51004)