pCMV-N2-6MYC-hMID2
(Plasmid #51035)

• Purpose: mammalian expression of myc-tagged human MID2
• Depositing Lab: Timothy Cox
• Publication: Short et al BMC Cell Biol. 2002;3:1. Epub 2002 Jan 4.

Backbone

• Vector backbone: pCMV-N2-6Myc
• Backbone manufacturer: Cox lab
• Modifications to backbone: Vector is modified from the pEGFP-N2 vector (Clontech, Palo Alto, CA). Briefly, the GFP coding region was excised from pEGFP-N2 with NotI and BamHI, the 5' overhangs filled using T4 DNA polymerase and the vector religated to give pCMV-N2. Six copies of the myc epitope containing a start codon was amplified from pGEM-6mycT (gift from M. Whitelaw, University of Adelaide), and cloned into the HindIII site of pCMV-N2.
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: MID2
• Species: H. sapiens (human)
• Entrez Gene: MID2 (a.k.a. FXY2, MRX101, RNF60, TRIM1)
• Promoter: CMV
• Tag / Fusion Protein:
  • MYC (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (not destroyed)
• 3′ cloning site: EcoRI (not destroyed)
• 5′ sequencing primer: CMV-F
• 3′ sequencing primer: SV40pA-R

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please note: hMID2 begins at residue 19 of the NCBI reference sequence.