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mEmerald-PCNA-19-SV40NLS-4
(Plasmid #54223)

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Full plasmid sequence is not available for this item.

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 54223 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    mEmerald
  • Backbone size w/o insert (bp) 4750
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    PCNA-19 SV40NLS
  • Alt name
    Human Proliferating Cell Nuclear Antigen
  • Alt name
    NM_002592.2
  • Species
    H. sapiens (human)
  • Entrez Gene
    PCNA (a.k.a. ATLD2)
  • Promoter CMV
  • Tags / Fusion Proteins
    • mEmerald (N terminal on backbone)
    • 3xSV40NLS (N terminal on backbone)

Cloning Information

Resource Information

Depositor Comments

Human Proliferating Cell Nuclear Antigen + SV40 NLS 3x Repeat. Excitation = 487; Emission = 509. Localization data: Nucleus

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    mEmerald-PCNA-19-SV40NLS-4 was a gift from Michael Davidson (Addgene plasmid # 54223)
  • For your References section:

    G2 phase chromatin lacks determinants of replication timing. Lu J, Li F, Murphy CS, Davidson MW, Gilbert DM. J Cell Biol. 2010 Jun 14;189(6):967-80. doi: 10.1083/jcb.201002002. Epub 2010 Jun 7. 10.1083/jcb.201002002 PubMed 20530209