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AAV-hSyn-Lyn-Y-GECO1
(Plasmid #58200)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 58200 Plasmid sent as bacteria in agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    AAV with hSyn promoter
  • Backbone size w/o insert (bp) 4563
  • Total vector size (bp) 5895
  • Vector type
    AAV

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Y-GECO1.0
  • Alt name
    A yellow fluorescent calcium ion indicator with inverted fluorescent response
  • Species
    Synthetic
  • Insert Size (bp)
    1332
  • GenBank ID
    KJ193859
  • Promoter hSyn

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site HindIII (not destroyed)
  • 5′ sequencing primer GAG GAG TCG TGT CGT GCC
  • 3′ sequencing primer CATAGCGTAAAAGGAGCAACA
  • (Common Sequencing Primers)

Resource Information

  • Terms and Licenses
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    AAV-hSyn-Lyn-Y-GECO1 was a gift from Robert Campbell (Addgene plasmid # 58200)
  • For your References section:

    Microfluidic cell sorter-aided directed evolution of a protein-based calcium ion indicator with an inverted fluorescent response. Zhao Y, Abdelfattah AS, Zhao Y, Ruangkittisakul A, Ballanyi K, Campbell RE, Harrison DJ. Integr Biol (Camb). 2014 May 19. 10.1039/c4ib00039k PubMed 24840546