Purpose(Empty Backbone) Baculovirus expression vector for secretion with N-terminal Honeybee melittin signal and C-terminal 8xHis tag
Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||62288||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size (bp) 10000
Modifications to backboneAdds one or two (A) amino acids after the signalase cleavage site before the 8xHis tag
Vector typeInsect Expression ; Baculovirus
- Promoter polyhedrin
/ Fusion Proteins
- 8XHis tag (N terminal on backbone)
- 8XHis tag (C terminal on backbone)
Growth in Bacteria
- Cloning method Unknown
- 5′ sequencing primer phF 5’ AGACGCACAAACTAATATCACAAACTGGA 3’
- 3′ sequencing primer mR 5’ CGTGTCGGGTTTAACATTACGGAT 3’ (Common Sequencing Primers)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pAB-LIC C was a gift from Cheryl Arrowsmith (Addgene plasmid # 62288 ; http://n2t.net/addgene:62288 ; RRID:Addgene_62288)