PurposeExpresses a non-targeting gRNA and Cre-recombinase
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||66895||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
- Total vector size (bp) 7761
Vector typeMammalian Expression, Mouse Targeting, Lentiviral, Cre/Lox, CRISPR
Growth in Bacteria
SpeciesM. musculus (mouse)
- Promoter U6
- Cloning method Restriction Enzyme
- 5′ cloning site SbfI (not destroyed)
- 3′ cloning site NotI (not destroyed)
- 5′ sequencing primer GGTACAGTGCAGGGGAAAGA (Common Sequencing Primers)
20bp non-targeting sgRNA sequence obtained from Sanjana et al. Nat Methods. 2014 Aug;11(8):783-4. doi: 10.1038/nmeth.3047.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:Lenti-sgNT/Cre was a gift from Monte Winslow (Addgene plasmid # 66895 ; http://n2t.net/addgene:66895 ; RRID:Addgene_66895)
For your References section:Pancreatic cancer modeling using retrograde viral vector delivery and in vivo CRISPR/Cas9-mediated somatic genome editing. Chiou SH, Winters IP, Wang J, Naranjo S, Dudgeon C, Tamburini FB, Brady JJ, Yang D, Gruner BM, Chuang CH, Caswell DR, Zeng H, Chu P, Kim GE, Carpizo DR, Kim SK, Winslow MM. Genes Dev. 2015 Jul 15;29(14):1576-85. doi: 10.1101/gad.264861.115. Epub 2015 Jul 15. 10.1101/gad.264861.115 PubMed 26178787