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pHKMC1: Empty sgRNA for Cloning
(Plasmid #67720)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 67720 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    U6::unc-119_sgRNA from J.Calarco (Plasmid #46169)
  • Backbone size (bp) 3479
  • Modifications to backbone
    unc-119 sgRNA sequence from PU6::unc-119_sgRNA(Plasmid #46169) was replaced by NotI and BamHI sites for sgRNA cloning purpose.
  • Vector type
    Worm Expression
  • Promoter PU6

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    NEB 5-alpha (DH5alpha derivative)
  • Copy number
    High Copy

Cloning Information

  • Cloning method Gibson Cloning

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pHKMC1: Empty sgRNA for Cloning was a gift from Monica Colaiacovo (Addgene plasmid # 67720 ; http://n2t.net/addgene:67720 ; RRID:Addgene_67720)
  • For your References section:

    Efficient Genome Editing in Caenorhabditis elegans with a Toolkit of Dual-Marker Selection Cassettes. Norris AD, Kim HM, Colaiacovo MP, Calarco JA. Genetics. 2015 Oct;201(2):449-58. doi: 10.1534/genetics.115.180679. Epub 2015 Jul 30. 10.1534/genetics.115.180679 PubMed 26232410