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AAV Cag Flex H2B Gcamp6s reverse
(Plasmid #74157)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 74157 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    AAV CAG Flex
  • Backbone size w/o insert (bp) 5112
  • Total vector size (bp) 6771
  • Vector type
    AAV

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    30°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    Gcamp6s
  • Alt name
    GCaMP3-K78H T302L R303P D380Y T381R S383T R392G
  • Species
    R. norvegicus (rat), G. gallus (chicken); ; A. victoria (jellyfish)
  • Insert Size (bp)
    1353
  • Promoter SpeI
  • Tag / Fusion Protein
    • H2B (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site SpeI (destroyed during cloning)
  • 3′ cloning site SpeI (destroyed during cloning)
  • 5′ sequencing primer AAV1145f; ctgtggctgcgtgaaagccttg
  • 3′ sequencing primer AAV1621r:ctgacaacgggccacaactc
  • (Common Sequencing Primers)

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    AAV Cag Flex H2B Gcamp6s reverse was a gift from Loren Looger (Addgene plasmid # 74157 ; http://n2t.net/addgene:74157 ; RRID:Addgene_74157)