14UAS zfPSD95:GFP
(Plasmid #74314)

• Purpose: A fusion of zebrafish PSD95 to GFP under control of 14 UAS repeats
• Depositing Labs: Martin Meyer, Stephen Smith
• Publication: Niell et al Nat Neurosci. 2004 Mar;7(3):254-60. Epub 2004 Feb 1.

Backbone

• Vector backbone: pEGFP-N2
• Backbone manufacturer: Clontech/Invitrogen
• Backbone size w/o insert (bp): 5
• Total vector size (bp): 7
• Modifications to backbone: CMV promoter removed and replaced with 14 UAS repeats

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: zebrafish PSD95
• Species: D. rerio (zebrafish)
• Insert Size (bp): 2406
• Mutation: Y599H and I793S (please see depositor comment below)
• Promoter: UAS
• Tag / Fusion Protein:
  • EGFP (C terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoR1 (not destroyed)
• 3′ cloning site: Sma1 (not destroyed)
• 5′ sequencing primer: ATGCCTCTCAAACGAGAAGA
• 3′ sequencing primer: GGATCCCAGCACGAGAGAGACTGTAA

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

The depositor noted that the Y599H and I793S mutations found in Addgene's quality control sequence does NOT affect plasmid function.