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14UAS PSD95:GFP 5UAS DSRedExpress
(Plasmid #74315)

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Full plasmid sequence is not available for this item.

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 74315 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pEGFP-N2
  • Backbone manufacturer
    clontech/invitrogen
  • Backbone size w/o insert (bp) 5
  • Total vector size (bp) 8
  • Modifications to backbone
    CMV replaced by 14 UAS repeats

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert 1

  • Gene/Insert name
    zebrafish PSD95
  • Species
    D. rerio (zebrafish)
  • Insert Size (bp)
    2406
  • Mutation
    Y599H and I793S (please see depositor comment below)
  • Tag / Fusion Protein
    • EGFP (C terminal on backbone)

Gene/Insert 2

  • Gene/Insert name
    DsRedExpress

Resource Information

Depositor Comments

The depositor noted that the Y599H and I793S mutations found in Addgene's quality control sequence does NOT affect plasmid function.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    14UAS PSD95:GFP 5UAS DSRedExpress was a gift from Martin Meyer & Stephen Smith (Addgene plasmid # 74315 ; http://n2t.net/addgene:74315 ; RRID:Addgene_74315)
  • For your References section:

    In vivo imaging of synapse formation on a growing dendritic arbor. Niell CM, Meyer MP, Smith SJ. Nat Neurosci. 2004 Mar;7(3):254-60. Epub 2004 Feb 1. 10.1038/nn1191 PubMed 14758365