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pFUChW Parkin shRNA Mul1 shRNA
(Plasmid #78523)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 78523 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pFUChW
  • Backbone size w/o insert (bp) 3963
  • Total vector size (bp) 4539
  • Modifications to backbone
    Additional H1 added
  • Vector type
    Lentiviral, RNAi

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Park2 shRNA, Mul1 shRNA
  • gRNA/shRNA sequence
    CCTCCAAGGAAACCATCAA, GAGCTGTGCGGTCTGTTAA
  • Species
    M. musculus (mouse)
  • Promoter H1

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI, XbaI (unknown if destroyed)
  • 3′ cloning site EcoRI, NheI (unknown if destroyed)
  • 5′ sequencing primer GTCGCTATGTGTTCTGGGAAATCACC
  • 3′ sequencing primer GTCGCTATGTGTTCTGGGAAATCACC
  • (Common Sequencing Primers)

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pFUChW Parkin shRNA Mul1 shRNA was a gift from David Chan (Addgene plasmid # 78523 ; http://n2t.net/addgene:78523 ; RRID:Addgene_78523)
  • For your References section:

    Elimination of paternal mitochondria in mouse embryos occurs through autophagic degradation dependent on PARKIN and MUL1. Rojansky R, Cha MY, Chan DC. Elife. 2016 Nov 17;5. pii: e17896. doi: 10.7554/eLife.17896. 10.7554/eLife.17896 PubMed 27852436