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Elimination of paternal mitochondria in mouse embryos occurs through autophagic degradation dependent on PARKIN and MUL1.
Rojansky R, Cha MY, Chan DC
Elife. 2016 Nov 17;5. pii: e17896. doi: 10.7554/eLife.17896.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
78518pBABEpuro mTurquoise2 LC3BFluorescent reporter for LC3B visualization
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78520pCLBW cox8 EGFP mCherryFluorescent mitophagy reporter
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78522pFUChW nontargeting shRNALentiviral vector encoding shRNA and mCherry reporter
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78523pFUChW Parkin shRNA Mul1 shRNALentiviral vector encoding two shRNAs and mCherry reporter
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Antibodies from Article