pZac2.1 EFs-SaCas9-U6-Sa DMDR7-U6-SaDMDL2
PurposeA single vector AAV-Cas9 system containing SaCas9 under EFs promoter, gRNAs targeting Dmd introns 22 and 23
Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||78607||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
- Total vector size (bp) 7581
Vector typeMammalian Expression, AAV, CRISPR
Growth in Bacteria
Growth Strain(s)NEB Stable
Gene/Insert nameEFs-SaCas9-U6-Sa DMDR7-U6-SaDMDL2
Insert Size (bp)4418
- Promoter EF1a-short; U6
/ Fusion Protein
- HA tag (C terminal on insert)
- Cloning method Gibson Cloning
- 5′ sequencing primer GAATTCGAGCTCGGTACCC
- 3′ sequencing primer TCGACTCTAGAGGATCCCC (Common Sequencing Primers)
A portion of this plasmid was derived from a plasmid made bySaCas9 from Feng Zhang at Broad Institute.
Terms and Licenses
gRNA targeting sequences:
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pZac2.1 EFs-SaCas9-U6-Sa DMDR7-U6-SaDMDL2 was a gift from Amy Wagers (Addgene plasmid # 78607 ; http://n2t.net/addgene:78607 ; RRID:Addgene_78607)
For your References section:In vivo gene editing in dystrophic mouse muscle and muscle stem cells. Tabebordbar M, Zhu K, Cheng JK, Chew WL, Widrick JJ, Yan WX, Maesner C, Wu EY, Xiao R, Ran FA, Cong L, Zhang F, Vandenberghe LH, Church GM, Wagers AJ. Science. 2016 Jan 22;351(6271):407-11. doi: 10.1126/science.aad5177. Epub 2015 Dec 31. 10.1126/science.aad5177 PubMed 26721686
Map uploaded by the depositor.