PurposeEncodes Cas9 and a CRISPR sgRNA that alleviates toxicity to Cas9 in Toxoplasma gondii
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||80324||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 8360
- Total vector size (bp) 8380
Modifications to backboneThe decoy protospacer has been cloned into the BsaI sites.
Growth in Bacteria
Gene/Insert nameDecoy sgRNA
- Promoter U6
- Cloning method Gibson Cloning
- 5′ sequencing primer GCCCATGGGATGAGACAAAG (Common Sequencing Primers)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pU6-Decoy was a gift from Sebastian Lourido (Addgene plasmid # 80324 ; http://n2t.net/addgene:80324 ; RRID:Addgene_80324)
For your References section:A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik SM, Huet D, Ganesan SM, Huynh MH, Wang T, Nasamu AS, Thiru P, Saeij JP, Carruthers VB, Niles JC, Lourido S. Cell. 2016 Sep 8;166(6):1423-1435.e12. doi: 10.1016/j.cell.2016.08.019. Epub 2016 Sep 2. 10.1016/j.cell.2016.08.019 PubMed 27594426