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Pvha6-AgDD
(Plasmid #80626)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 80626 Plasmid sent as bacteria in agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    modified worm expression vector with Pvha6 promoter
  • Backbone size w/o insert (bp) 4607
  • Total vector size (bp) 5697
  • Modifications to backbone
    same insert as Plasmid #78289 (AgDD), but with with Pvha6 promoter for worm expression.
  • Vector type
    Worm Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    NES-L106P-sfGFP
  • Alt name
    AgDD
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1087
  • GenBank ID
    NM_000801 AB971579
  • Tag / Fusion Protein
    • MLALKLAGLDI (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site NcoI (not destroyed)
  • 5′ sequencing primer unknown
  • 3′ sequencing primer unknown
  • (Common Sequencing Primers)

Resource Information

Depositor Comments

The depositor-provided reference sequence shows 3 additional nucleotide mismatches compared to listed NM_000801 and also F36V compared to the respective protein (NP_000792.1). Addgene Sanger sequencing confirmed these differences.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Pvha6-AgDD was a gift from Thomas Wandless (Addgene plasmid # 80626)
  • For your References section:

    A method to rapidly create protein aggregates in living cells. Miyazaki Y, Mizumoto K, Dey G, Kudo T, Perrino J, Chen LC, Meyer T, Wandless TJ. Nat Commun. 2016 May 27;7:11689. doi: 10.1038/ncomms11689. 10.1038/ncomms11689 PubMed 27229621