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mCherry-FDD
(Plasmid #80629)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 80629 Plasmid sent as bacteria in agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pBMN
  • Backbone manufacturer
    Gary Nolan
  • Backbone size w/o insert (bp) 6940
  • Total vector size (bp) 7190
  • Modifications to backbone
    Blastcidin-S resistanct gene (bsr) behind IRES
  • Vector type
    Retroviral
  • Selectable markers
    Blasticidin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    30°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    UnaG
  • Species
    Anguilla japonica
  • Insert Size (bp)
    1216
  • Mutation
    mutations A36V, R136G
  • GenBank ID
    AB763906
  • Promoter LTR
  • Tag / Fusion Protein
    • HA-mcherry-linker (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site NotI (not destroyed)
  • 5′ sequencing primer Lab32 ATCGCAGCTTGGATACACGCC
  • 3′ sequencing primer LC54 CATATAGACAAACGCACACCGGCCTTA
  • (Common Sequencing Primers)

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    mCherry-FDD was a gift from Thomas Wandless (Addgene plasmid # 80629)
  • For your References section:

    A Novel Destabilizing Domain Based on a Small-Molecule Dependent Fluorophore. Navarro R, Chen LC, Rakhit R, Wandless TJ. ACS Chem Biol. 2016 Jun 6. 10.1021/acschembio.6b00234 PubMed 27243964