Addgene - p142 Plasmid Data

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8385: p142
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Plasmid 8385: p142

Gene/insert name:
LOX I/LOX II
Insert size:
36
Species:
P1 bacteriophage
Fusion protein or tag:
EGFP
Terminal:
N terminal on backbone
Vector backbone:
pEGFP-C1
(Search Vector Database)
Vector type:
Mammalian Expression, Cre/Lox
Backbone size w/o insert:
4700
Cloning site 5':
MluI
Site destroyed during cloning:
Yes
Cloning site 3':
DraIII
Site destroyed during cloning:
No
5' sequencing primer:
na List of Sequencing Primers
Bacterial resistance(s)
Kanamycin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Selectable markers:
Neomycin
Sequence: View sequences (1)
Map:
View map
Principal Investigator:
Jeffrey Green
Terms and Licenses:
MTA

Comments:

EGFP flanked by LOX sites. Derived from plasmid p138.

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: A single vector containing modified cre recombinase and LOX recombination sequences for inducible tissue-specific amplification of gene expression. Kaczmarczyk et al (Nucleic Acids Res 2001 Jun 15;29(12):E56-6. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 8385" in your Materials and Methods section.