1
Plasmid 8450: Retrohair
  • None

  • pBABE
    (Search Vector Database)

  • Mammalian Expression, Retroviral, RNAi

  • AgeI

  • No

  • EcoRI

  • No

  • pLXSN 5' List of Sequencing Primers

  • Ampicillin

  • DH5alpha

  • 37

  • High Copy

  • Puromycin

  • View sequences (1)
  • Weinberg-viral (application/pdf)

  • Bob Weinberg

  • UBMTA

Comments: 

Empty Moloney murine leukemia virus (MuLV) retroviral vector for siRNA expression, see also pMKO.1. The 293T cell line can be obtained from the Weinberg lab or GenHunter http://genhunter.com/products/aptag-3/index.html

Addgene has sequenced a portion of this plasmid for verification. Full plasmid sequence is available only if provided by the depositing laboratory.

Article: Lentivirus-delivered stable gene silencing by RNAi in primary cells. Stewart et al (RNA 2003 Apr;9(4):493-501. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 8450" in your Materials and Methods section.

Price: US $65

Available to academic and non-profits only