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pAAV-U6-sgRNA-CMV-GFP
(Plasmid #85451)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 85451 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    pCMV-EGFP
  • gRNA/shRNA sequence
    for cloning by SapI
  • Promoter pCMV-EGFP

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XbaI (not destroyed)
  • 3′ cloning site EcoR1 (not destroyed)
  • 5′ sequencing primer cgTCTAGAgGGTACCgGGGCCCgGTCGAC tagttattaa tagtaatcaa ttacgg
  • 3′ sequencing primer caGAATTCgC TGC AGg TTA TCG AGA TCT GAG TCC GGA CTT GTA
  • (Common Sequencing Primers)

Resource Information

Depositor Comments

We PCR amplified p-CMV-EGFR from the pEGFP-C1 vector and cloned it into the 60958 vector from Addgene

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAAV-U6-sgRNA-CMV-GFP was a gift from Hetian Lei (Addgene plasmid # 85451 ; http://n2t.net/addgene:85451 ; RRID:Addgene_85451)
  • For your References section:

    The CRISPR/Cas9-created MDM2 T309G enhances vitreous-induced expression of MDM2 and proliferation and survival of cells. Duan Y, Ma G, Huang X, D'Amore PA, Zhang F, Lei H. J Biol Chem. 2016 May 31. pii: jbc.M116.729467. 10.1074/jbc.M116.729467 PubMed 27246850