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pTol-Promoter:NLS-tdMCP-eGFP
(Plasmid #86248)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 86248 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pTolDestR4-R2pA
  • Backbone manufacturer
    Villefranc et al., 2007
  • Modifications to backbone
    The βactin (Higashijima et al., 1997) and hsp70l (Halloran et al., 2000) promoter 5′ entry clones (p5E) were previously described (Kwan et al., 2007). Appropriate p5E and pME plasmids were recombined with pTolDestR4-R2pA (Villefranc et al., 2007) following the manufacturer’s instructions (Invitrogen) to make pTolBetaActin: NLS-tdMCP-eGFP.
  • Vector type
    Used with transposase to make transient or stable transgenic zebrafish lines

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    beta Actin promoter:NLS-tdMCPeGFP
  • Tag / Fusion Protein
    • NLS-tdMCP-eGFP (C terminal on insert)

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pTol-Promoter:NLS-tdMCP-eGFP was a gift from Florence Marlow (Addgene plasmid # 86248 ; http://n2t.net/addgene:86248 ; RRID:Addgene_86248)
  • For your References section:

    Dynamic visualization of transcription and RNA subcellular localization in zebrafish. Campbell PD, Chao JA, Singer RH, Marlow FL. Development. 2015 Apr 1;142(7):1368-74. doi: 10.1242/dev.118968. Epub 2015 Mar 10. 10.1242/dev.118968 PubMed 25758462