pcDNA3 tdTomato LIC cloning vector (6F)
(Plasmid #86963)

• Purpose: (Empty Backbone) Empty LIC vector; adds a tdTomato gene to the C-terminus of your protein of interest.
• Depositing Lab: Chris Jeans
• Publication: 6-series mammalian LIC expression vectors (unpublished)

Backbone

• Vector backbone: pcDNA3
• Backbone size (bp): 6859
• Vector type: Mammalian Expression
• Promoter: CMV
• Selectable markers: Neomycin (select with G418)
• Tag / Fusion Protein:
  • tdTomato (C terminal on backbone)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Cloning Information

• Cloning method: Ligation Independent Cloning
• 5′ sequencing primer: CMV F (5'cgcaaatgggcggtaggcgtg)

Resource Information

• Supplemental Documents:
  • 6F.gb
• A portion of this plasmid was derived from a plasmid made by: The sequence for tdTomato came from Addgene #62726 (pAAV-Syn-Chronos-tdTomato).

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry

Depositor Comments

This is an empty LIC vector derived from pcDNA3. It adds an tdTomato gene to the C-terminus of your protein of interest.

To clone into this vector, add the following tags to your PCR primers:

LIC vKoz Forward tag 5’-TACTTCCAATCCAATGCCACC(ATG)

LIC vGFP Reverse tag 5’-CTCCCACTACCAATGCC

Do NOT include a stop codon with your reverse primer.

T4-treat PCR with dCTP. Linearize vector with SspI, then T4-treat with dGTP. Can verify the presence of insert by digesting with HindIII and XbaI.

For more information, please see our website:
http://qb3.berkeley.edu/qb3/macrolab/