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pKM596
(Plasmid #8837)

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Available to Academic and Nonprofits Only

Backbone

  • Vector backbone
    pMal-C2
  • Backbone manufacturer
    New England Biolabs
  • Backbone size (bp) 6700
  • Modifications to backbone
    DNA encoding presumptive N-terminal signal peptide deleted.
  • Vector type
    Bacterial Expression
  • Tag / Fusion Protein
    • E. coli MBP (N terminal on backbone)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DB5
  • Growth instructions
    Because it carries a gene (ccdB ) that encodes a lethal DNA gyrase poison, this vector must be propogated in an E. coli gyrA mutant such as DB3 or DB5 (Invitrogen), which is immune to the action of CcdB.
  • Copy number
    Low Copy

Sequence Information

Cloning Information

Resource Information

  • Terms and Licenses

Depositor Comments

A Gateway destination vector for the production of recombinant proteins as fusions to the C-terminus of E. coli maltose-binding protein. Presumptive N-terminal signal peptide deleted.

How to cite this plasmid

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pKM596 was a gift from David Waugh (Addgene plasmid # 8837)
  • For your References section:

    Maltodextrin-binding proteins from diverse bacteria and archaea are potent solubility enhancers. Fox JD, Routzahn KM, Bucher MH, Waugh DS. FEBS Lett. 2003 Feb 27. 537(1-3):53-7. 10.1016/S0014-5793(03)00070-X PubMed 12606030