Addgene - pKM596 Plasmid Data

Plasmid 8837: pKM596

Gene/insert name:
maltose binding protein
Alt name:
MBP
Alt name:
MalE
Insert size:
1200
Species:
E. coli
Fusion protein or tag:
Gateway linker
Terminal:
C terminal on insert
Mutation:
DNA encoding natural N-termninal signal peptide deleted
Vector backbone:
pMal-C2
(Search Vector Database)
Backbone manufacturer:
New England Biolabs
Vector type:
Bacterial Expression
Backbone size w/o insert (bp):
6700
5' sequencing primer:
tac promoter List of Sequencing Primers
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DB5
Growth temperature (℃):
37
Growth instructions:
Because it carries a gene (ccdB ) that encodes a lethal DNA gyrase poison, this vector must be propogated in an E. coli gyrA mutant such as DB3 or DB5 (Invitrogen), which is immune to the action of CcdB.
High or low copy:
Low Copy
Sequence: View sequences (2)
Principal Investigator:
David Waugh
Terms and Licenses:
MTA

Comments:

A Gateway

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Maltodextrin-binding proteins from diverse bacteria and archaea are potent solubility enhancers. Fox et al (FEBS Lett. 2003 Feb 27. 537(1-3):53-7. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 8837" in your Materials and Methods section.

Price: $65.00

Available to academic and non-profits only

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This is commonly requested with

11085: pDEST-HisMBP

11086: pDEST-periHisMBP

11520: pDest-565

8838: pKM839

8831: pRK603