1
Plasmid 8837: pKM596
  • None

  • pMal-C2
    (Search Vector Database)

  • New England Biolabs

  • Bacterial Expression

  • 6700

  • DNA encoding presumptive N-terminal signal peptide deleted.

  • MBP-F List of Sequencing Primers

  • M13_pUC_fwd; M13-F20

  • E. coli MBP

  • N terminal on backbone

  • Ampicillin

  • DB5

  • 37

  • Because it carries a gene (ccdB ) that encodes a lethal DNA gyrase poison, this vector must be propogated in an E. coli gyrA mutant such as DB3 or DB5 (Invitrogen), which is immune to the action of CcdB.

  • Low Copy

  • View sequences (2)
  • David Waugh

  • UBMTA

Comments: 

A Gateway destination vector for the production of recombinant proteins as fusions to the C-terminus of E. coli maltose-binding protein. Presumptive N-terminal signal peptide deleted.

Addgene has sequenced a portion of this plasmid for verification. Full plasmid sequence is available only if provided by the depositing laboratory.

Article: Maltodextrin-binding proteins from diverse bacteria and archaea are potent solubility enhancers. Fox et al (FEBS Lett. 2003 Feb 27. 537(1-3):53-7. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 8837" in your Materials and Methods section.

Price: US $65

Available to academic and non-profits only