|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||8837||Plasmid sent as bacteria in agar stab||1||$65|
This material is available to academics and nonprofits only.
Backbone manufacturerNew England Biolabs
- Backbone size (bp) 6700
Modifications to backboneDNA encoding presumptive N-terminal signal peptide deleted.
Vector typeBacterial Expression
/ Fusion Protein
- E. coli MBP (N terminal on backbone)
Growth in Bacteria
Growth instructionsBecause it carries a gene (ccdB ) that encodes a lethal DNA gyrase poison, this vector must be propogated in an E. coli gyrA mutant such as DB3 or DB5 (Invitrogen), which is immune to the action of CcdB.
Copy numberLow Copy
- Cloning method Gateway Cloning
- 5′ sequencing primer MBP-F
- 3′ sequencing primer M13_pUC_fwd; M13-F20 (Common Sequencing Primers)
A Gateway destination vector for the production of recombinant proteins as fusions to the C-terminus of E. coli maltose-binding protein. Presumptive N-terminal signal peptide deleted.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pKM596 was a gift from David Waugh (Addgene plasmid # 8837)
For your References section:Maltodextrin-binding proteins from diverse bacteria and archaea are potent solubility enhancers. Fox JD, Routzahn KM, Bucher MH, Waugh DS. FEBS Lett. 2003 Feb 27. 537(1-3):53-7. 10.1016/S0014-5793(03)00070-X PubMed 12606030