Addgene - pKM1137 Plasmid Data

Plasmid 8841: pKM1137

Gene/insert name:
maltose binding protein
Alt name:
MBP
Insert size:
1200
Species:
V. cholera
Fusion protein or tag:
Gateway Linker
Terminal:
C terminal on insert
Mutation:
DNA encoding presumptive N-terminal signal peptide deleted.
Vector backbone:
pET11
(Search Vector Database)
Backbone manufacturer:
Novagen
Vector type:
Bacterial Expression
Backbone size w/o insert (bp):
6700
5' sequencing primer:
T7 List of Sequencing Primers
Bacterial resistance(s)
Ampicillin
Growth strain(s)
ccdB Survival
Growth temperature (℃):
37
Growth instructions:
Because it carries a gene (ccdB ) that encodes a lethal DNA gyrase poison, this vector must be propogated in an E. coli gyrA mutant such as DB3 or DB5 (Invitrogen), which is immune to the action of CcdB.
High or low copy:
Low Copy
Sequence: View sequences (2)
Principal Investigator:
David Waugh
Terms and Licenses:
MTA
EMD Millipore Limited Use Label License/Brookhaven pET Assurance Letter

Comments:

A Gateway

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Maltodextrin-binding proteins from diverse bacteria and archaea are potent solubility enhancers. Fox et al (FEBS Lett. 2003 Feb 27. 537(1-3):53-7. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 8841" in your Materials and Methods section.

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