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pAAV-U6-CB2gRNA-CBh-mCherry
(Plasmid #91948)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 91948 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    AAV:ITR-U6-sgRNA(backbone)-pCBh-Cre-WPRE-hGHpA-ITR
  • Backbone manufacturer
    F. Zhang Lab (Addgene #60229)
  • Backbone size w/o insert (bp) 6394
  • Total vector size (bp) 5956
  • Modifications to backbone
    1. The Cre gene in the backbone plasmid was replaced by the mCherry gene at the restriction sites AgeI and EcoRI. The mCherry DNA was PCR-amplified from the plasmid pAAV-CaMKIIa-hChR2(E123T/T159C)-mCherry (Addgene #35512). 2. The 17-bp scrambled gRNA sequence between the U6 promoter and gRNA scaffolding sequence in the control plasmid was replaced by 19-bp CB2R gRNA sequence, which targets the early part of CB2R coding sequence (26th–44th bp of 1044 bp). gRNA sequence: 5' GTGACCAACGGCTCCAACGG 3'
  • Vector type
    AAV

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    mCherry
  • Species
    Synthetic
  • Insert Size (bp)
    711

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAAV-U6-CB2gRNA-CBh-mCherry was a gift from Jimok Kim (Addgene plasmid # 91948 ; http://n2t.net/addgene:91948 ; RRID:Addgene_91948)
  • For your References section:

    Distinct roles of neuronal and microglial CB2 cannabinoid receptors in the mouse hippocampus. Li Y, Kim J. Neuroscience. 2017 Sep 6. pii: S0306-4522(17)30629-2. doi: 10.1016/j.neuroscience.2017.08.053. 10.1016/j.neuroscience.2017.08.053 PubMed 28888955