PurposeExpression plasmid for both MS2-P65-HSF1 activator helper complex and sgRNA with two MS2 loops at tetraloop and stemloop 2 contains BsaI sites for insertion of spacer sequences.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||92120||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Total vector size (bp) 6140
Vector typeMammalian Expression, CRISPR
Growth in Bacteria
Growth Strain(s)NEB Stable
Copy numberHigh Copy
SpeciesH. sapiens (human), Synthetic
Insert Size (bp)1428
Entrez GeneHSF1 (a.k.a. HSTF1)
- Promoter EF1a
- Cloning method Unknown
- 5′ sequencing primer CCTCTGACTTGAGCGTCG (Common Sequencing Primers)
U6-sgRNA(MS2)_EF1a-MS2-P65-HSF1 is an altered version of the #61424 and #61423 plasmids from the Feng Zhang lab.
This plasmid makes it easier to transiently express the MS2 containing sgRNA and the MS2-P65-HSF1 activator helper complex in cell lines.
For detailed information and plasmid usage, please see the associated publication.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:U6-sgRNA(MS2)_EF1a-MS2-P65-HSF1 was a gift from Ervin Welker (Addgene plasmid # 92120 ; http://n2t.net/addgene:92120 ; RRID:Addgene_92120)
For your References section:Crossing enhanced and high fidelity SpCas9 nucleases to optimize specificity and cleavage. Kulcsar PI, Talas A, Huszar K, Ligeti Z, Toth E, Weinhardt N, Fodor E, Welker E. Genome Biol. 2017 Oct 6;18(1):190. doi: 10.1186/s13059-017-1318-8. 10.1186/s13059-017-1318-8 [pii] PubMed 28985763