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U6-sgRNA(MS2)_EF1a-MS2-P65-HSF1
(Plasmid #92120)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 92120 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    MS2-P65-HSF1
  • Species
    H. sapiens (human), Synthetic
  • Insert Size (bp)
    1428
  • Entrez Gene
    HSF1 (a.k.a. HSTF1)
  • Promoter EF1a

Cloning Information

Resource Information

  • Terms and Licenses

Depositor Comments

U6-sgRNA(MS2)_EF1a-MS2-P65-HSF1 is an altered version of the #61424 and #61423 plasmids from the Feng Zhang lab.
This plasmid makes it easier to transiently express the MS2 containing sgRNA and the MS2-P65-HSF1 activator helper complex in cell lines.

For detailed information and plasmid usage, please see the associated publication.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    U6-sgRNA(MS2)_EF1a-MS2-P65-HSF1 was a gift from Ervin Welker (Addgene plasmid # 92120 ; http://n2t.net/addgene:92120 ; RRID:Addgene_92120)
  • For your References section:

    Crossing enhanced and high fidelity SpCas9 nucleases to optimize specificity and cleavage. Kulcsar PI, Talas A, Huszar K, Ligeti Z, Toth E, Weinhardt N, Fodor E, Welker E. Genome Biol. 2017 Oct 6;18(1):190. doi: 10.1186/s13059-017-1318-8. 10.1186/s13059-017-1318-8 [pii] PubMed 28985763