U6-sgRNA(MS2)_EF1a-MS2-P65-HSF1
(Plasmid #92120)

• Purpose: Expression plasmid for both MS2-P65-HSF1 activator helper complex and sgRNA with two MS2 loops at tetraloop and stemloop 2 contains BsaI sites for insertion of spacer sequences.
• Depositing Lab: Ervin Welker
• Publication: Kulcsar et al Genome Biol. 2017 Oct 6;18(1):190. doi: 10.1186/s13059-017-1318-8.

Backbone

• Vector backbone: pX330-like
• Total vector size (bp): 6140
• Vector type: Mammalian Expression, CRISPR

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: MS2-P65-HSF1
• Species: H. sapiens (human), Synthetic
• Insert Size (bp): 1428
• Entrez Gene: HSF1 (a.k.a. HSTF1)
• Promoter: EF1a

Cloning Information

• Cloning method: Unknown
• 5′ sequencing primer: CCTCTGACTTGAGCGTCG

Resource Information

• Articles Citing this Plasmid:
  • 2 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

U6-sgRNA(MS2)_EF1a-MS2-P65-HSF1 is an altered version of the #61424 and #61423 plasmids from the Feng Zhang lab.
This plasmid makes it easier to transiently express the MS2 containing sgRNA and the MS2-P65-HSF1 activator helper complex in cell lines.

For detailed information and plasmid usage, please see the associated publication.