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The Rag GTPases bind raptor and mediate amino acid signaling to mTORC1.
Sancak Y, Peterson TR, Shaul YD, Lindquist RA, Thoreen CC, Bar-Peled L, Sabatini DM
Science. 2008 Jun 13. 320(5882):1496-501.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
19298pRK5-HA GST RagA wt
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19299pRK5-HA GST RagA 21L
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19300pRK5-HA GST RagA 66L
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19301pRK5-HA GST RagB wt
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19302pRK5-HA GST RagB 54L
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19303pRK5-HA GST RagB 99L
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19304pRK5-HA GST RagC wt
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19305pRK5-HA GST RagC 75L
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19306pRK5-HA GST RagC 120L
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19307pRK5-HA GST RagD wt
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19308pRK5-HA GST RagD 77L
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19309pRK5-HA GST RagD 121L
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19310pRK5-HA GST Rheb1
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19311Flag pLJM1 Rap2A
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19312Flag pLJM1 Rheb1
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19313Flag pLJM1 RagB wt
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19314Flag pLJM1 RagB 54L
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19315Flag pLJM1 RagB 99L
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19316Flag pLJM1 RagD wt
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19317Flag pLJM1 RagD 77L
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19318Flag pLJM1 RagD 121L
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19319pLJM1-EGFP3rd gen lentiviral vector for EGFP fusion; PGK driven puromycin
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26625pLKO.1 Rheb1 shRNA #1
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26626pLKO.1 Rheb1 shRNA #2
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26627pLKO.1 RagB shRNA #1
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26628pLKO.1 RagB shRNA #2
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26629pLKO.1 RagC shRNA #1
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26630pLKO.1 RagC shRNA #2
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30319pLKO.1 RagA shRNA #1
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30320pLKO.1 RagA shRNA #2
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30321pLKO.1 RagD shRNA #1
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30322pLKO.1 RagD shRNA #2
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30323pLKO.1 GFP shRNA
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Antibodies from Article