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LuTHy: a double‐readout bioluminescence‐based two‐hybrid technology for quantitative mapping of protein–protein interactions in mammalian cells
Trepte P, Kruse S, Kostova S, Hoffmann S, Buntru A, Tempelmeier A, Secker C, Diez L, Schulz A, Klockmeier K, Zenkner M, Golusik S, Rau K, Schnoegl S, Garner CC, Wanker EE.
Molecular Systems Biology (2018) 14, e8071
Article

Plasmids from Article

ID Plasmid Purpose  
113442pcDNA3.1 NLNanoLuc control expression vector
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113443pcDNA3.1 PA-mCitProteinA-mCitrine control expression vector
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113444pcDNA3.1 PA-mCit-NLProteinA-mCitrine-NanoLuc hybrid-fusion control expression vector (positive control)
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113445pcDNA3.1 PA-NLProteinA-NanoLuc hybrid fusion control expression vector (for BRET and LuC normalization)
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113446pcDNA3.1 myc-NL-GWNanoLuc Gateway shuttle vector for N-terminal fusions
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113447pcDNA3.1 GW-NL-mycNanoLuc Gateway shuttle vector for C-terminal fusions
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113448pcDNA3.1 PA-mCit-GWProteinA-mCitrine Gateway shuttle vector for N-terminal fusions
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113449pcDNA3.1 GW-mCit-PAProteinA-mCitrine Gateway shuttle vector for C-terminal fusions
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113450pLenti NLLentiviral NanoLuc control expression vector
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113451pLenti PA-mCitLentiviral ProteinA-mCitrine expression vector
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113452pLenti PA-mCit-NLLentiviral ProteinA-mCitrine-NanoLuc hybrid-fusion control expression vector (positive control)
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113453pLenti PA-NLLentiviral ProteinA-NanoLuc hybrid fusion control expression vector (for BRET and LuC normalization)
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113454pLenti myc-NL-GWLentiviral NanoLuc Gateway shuttle vector for N-terminal fusions
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113455pLenti GW-NL-mycLentiviral NanoLuc Gateway shuttle vector for C-terminal fusions
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113456pLenti PA-mCit-GWLentiviral ProteinA-mCitrine Gateway shuttle vector for N-terminal fusions
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113457pLenti GW-mCit-PALentiviral ProteinA-mCitrine Gateway shuttle vector for C-terminal fusions
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113458pcDNA3.1 NL-BCL2L1NanoLuc-BCL2L1 expression vector (positive control together with pcDNA3.1 PA-mCit-BAD)
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113459pcDNA3.1 PA-mCit-BADProteinA-mCitrine-BAD expression vector (positive control together with pcDNA3.1 NL-BCL2L1)
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