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The shieldin complex mediates 53BP1-dependent DNA repair.
Noordermeer SM, Adam S, Setiaputra D, Barazas M, Pettitt SJ, Ling AK, Olivieri M, Alvarez-Quilon A, Moatti N, Zimmermann M, Annunziato S, Krastev DB, Song F, Brandsma I, Frankum J, Brough R, Sherker A, Landry S, Szilard RK, Munro MM, McEwan A, Goullet de Rugy T, Lin ZY, Hart T, Moffat J, Gingras AC, Martin A, van Attikum H, Jonkers J, Lord CJ, Rottenberg S, Durocher D
Nature. 2018 Aug;560(7716):117-121. doi: 10.1038/s41586-018-0340-7. Epub 2018 Jul 18.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
114115pcDNA5-FRT/TO-FLAG-SHLD1Expresses N-terminally tagged FLAG-SHLD1 in mammalian cells
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114116pCW-eGFP-SHLD1Lentiviral vector for inducible expression of N-terminally tagged eGFP-SHLD1
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114117pcDNA5-FRT/TO-FLAG-SHLD2Expresses N-terminally tagged FLAG-SHLD2 in mammalian cells
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114118pGLUE-SHLD2Expresses N-terminally tagged Streptag-HA-CBP-SHLD2 in mammalian cells
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114119pCW-eGFP-SHLD2Lentiviral vector for inducible expression of N-terminally tagged eGFP-SHLD2
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114120pCW-eGFP-SHLD2-m1Lentiviral vector for inducible expression of N-terminally tagged eGFP-SHLD2, m1 mutant
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114121pCW-eGFP-FHA-SHLD2Lentiviral vector for inducible expression of N-terminally tagged eGFP-RNF8 FHA domain-SHLD2; 53BP1-independent recruitment to damaged chromatin
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114122pCW-eGFP-FHA-SHLD2-m1Lentiviral vector for inducible expression of N-terminally tagged eGFP-RNF8 FHA domain-SHLD2, m1 mutant; 53BP1-independent recruitment to damaged chromatin
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114123pcDNA5-FRT/TO-FLAG-SHLD3Expresses N-terminally tagged FLAG-SHLD3 in mammalian cells
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114124pcDNA5-FRT/TO-V5-SHLD3Expresses N-terminally tagged V5-SHLD3 in mammalian cells
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114125pcDNA5-FRT/TO-eGFP-SHLD3Expresses N-terminally tagged eGFP-SHLD3 in mammalian cells
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114126pCW-eGFP-SHLD3Lentiviral vector for inducible expression of N-terminally tagged eGFP-tagged SHLD3
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114127pDEST-FRT/TO-3xFLAG-REV7Expresses N-terminally tagged 3x FLAG-REV7 in mammalian cells
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114128pDEST-FRT/TO-eGFP-REV7Expresses N-terminally tagged eGFP-REV7 in mammalian cells
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114129pCW-eGFP-FHALentiviral vector for inducible expression of eGFP-RNF8 FHA domain fusion; 53BP1-independent recruitment to damaged chromatin
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