A hydride transfer complex reprograms NAD metabolism and bypasses senescence.
Igelmann S, Lessard F, Uchenunu O, Bouchard J, Fernandez-Ruiz A, Rowell MC, Lopes-Paciencia S, Papadopoli D, Fouillen A, Ponce KJ, Huot G, Mignacca L, Benfdil M, Kalegari P, Wahba HM, Pencik J, Vuong N, Quenneville J, Guillon J, Bourdeau V, Hulea L, Gagnon E, Kenner L, Moriggl R, Nanci A, Pollak MN, Omichinski JG, Topisirovic I, Ferbeyre G
Mol Cell. 2021 Sep 16;81(18):3848-3865.e19. doi: 10.1016/j.molcel.2021.08.028. PubMed Article
Igelmann S, Lessard F, Uchenunu O, Bouchard J, Fernandez-Ruiz A, Rowell MC, Lopes-Paciencia S, Papadopoli D, Fouillen A, Ponce KJ, Huot G, Mignacca L, Benfdil M, Kalegari P, Wahba HM, Pencik J, Vuong N, Quenneville J, Guillon J, Bourdeau V, Hulea L, Gagnon E, Kenner L, Moriggl R, Nanci A, Pollak MN, Omichinski JG, Topisirovic I, Ferbeyre G
Mol Cell. 2021 Sep 16;81(18):3848-3865.e19. doi: 10.1016/j.molcel.2021.08.028. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 184465 | pUltra-MDH1-ME1 | Lentiviral vector for tri-cistronic expression of EGFP, MDH1 and ME1 (seperated by P2A and T2A) |
| 184466 | pUltra-Hot-PC | Lentiviral vector for bi-cistronic expression of mCherry and PC (seperated by P2A) |
| 184549 | pLPC-puromycin-binary-MDH1-3xFLAG-ME1- HA | Retroviral vector to co-express human MDH1 with 3xFLAG tag and human ME1 with HA tag |
| 184550 | pBABE-neomycin-PC-MYC | Retroviral vector to express human PC with MYC tag |
| 184558 | pHIS-MDH1-HIS | Bacterial expression of human MDH1 with HIS Tag |