Nonmonotone invasion landscape by noise-aware control of metastasis activator levels.
Wan Y, Cohen J, Szenk M, Farquhar KS, Coraci D, Krzyszton R, Azukas J, Van Nest N, Smashnov A, Chern YJ, De Martino D, Nguyen LC, Bien H, Bravo-Cordero JJ, Chan CH, Rosner MR, Balazsi G
Nat Chem Biol. 2023 Jul;19(7):887-899. doi: 10.1038/s41589-023-01344-z. Epub 2023 May 25. PubMed Article
Wan Y, Cohen J, Szenk M, Farquhar KS, Coraci D, Krzyszton R, Azukas J, Van Nest N, Smashnov A, Chern YJ, De Martino D, Nguyen LC, Bien H, Bravo-Cordero JJ, Chan CH, Rosner MR, Balazsi G
Nat Chem Biol. 2023 Jul;19(7):887-899. doi: 10.1038/s41589-023-01344-z. Epub 2023 May 25. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 199212 | LPUtopia-7 | Landing Pad Utopia_v7 donor plasmid for AAVS1 site-specific integration in human cells. Use NeoR and eGFP as positive selection marker for AAVS1-targeted insertion and RFP as negative selection marker |
| 199213 | pCAG-eSpCas9_sgAAVS1 | All-in-one plasmid encoding eSpCas9 and sgRNA targeting the AAVS1 site in human cells. |
| 199214 | pCMV-BACH1t_RFP | BACH1 C-terminus truncated isoform - BACH1t expression plasmid with a RFP reporter on the backbone. |
| 199215 | pCMV-RKIP_RFP | RKIP (PEBP1) expression plasmid with a RFP reporter on the backbone. |
| 199216 | pUC_hU6-shRKIP_BFP | Expression plasmid with shRNAmiR targeting RKIP (PEBP1), with a BFP reporter on the backbone. |
| 199217 | pUC-hU6-shBACH1_BFP | Expression plasmid with shRNAmiR targeting BACH1, with a BFP reporter on the backbone. |
| 199218 | pUt-mNF-BACH1 Cas9-Resistant | LPUtopia-7 matching RMCE donor plasmid with Cas9-resistant GFP-BACH1 Negative-Feedback circuit (mNF-BACH1 Cas9- resistant). Use BlastR for positive selection and HSV-TK for negative selection. |
| 199219 | pUt-mNF-BACH1 | LPUtopia-7 matching RMCE donor plasmid with GFP-BACH1 Negative-Feedback circuit (mNF-BACH1). Use BlastR for positive selection and HSV-TK for negative selection. |
| 199220 | pUt-mNF-GFP | LPUtopia-7 matching RMCE donor plasmid with GFP Negative-Feedback circuit (mNF-GFP). Use BlastR for positive selection and HSV-TK for negative selection. |
| 199221 | pUt-mPF-BACH1 Cas9-Resistant_AmpR | LPUtopia-7 matching RMCE donor plasmid with Cas9-resistant GFP-BACH1 Positive-Feedback circuit (mPF-BACH1). Use BlastR for positive selection and HSV-TK for negative selection. |
| 199222 | pUt-mPF-GFP | LPUtopia-7 matching RMCE donor plasmid with GFP Positive-Feedback circuit (mPF-GFP). Use BlastR for positive selection and HSV-TK for negative selection. |