Binary recombinase systems for high-resolution conditional mutagenesis.
Hermann M, Stillhard P, Wildner H, Seruggia D, Kapp V, Sanchez-Iranzo H, Mercader N, Montoliu L, Zeilhofer HU, Pelczar P
Nucleic Acids Res. 2014 Jan 9. PubMed Article
Hermann M, Stillhard P, Wildner H, Seruggia D, Kapp V, Sanchez-Iranzo H, Mercader N, Montoliu L, Zeilhofer HU, Pelczar P
Nucleic Acids Res. 2014 Jan 9. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 51263 | hGFAP-Roxed-Cre | Expression of the Dre-dependent Roxed-Cre gene under the control of the human GFAP promoter |
| 51264 | pCAG-attBr-Cre-rc-attP | Component of the Bxb1-rcCre binary site-specific recombinase system, reverse complementary Cre CDS flanked by Bxb1 attB/P recognition sites in head-to-head orientation |
| 51265 | pCAG-attBr-ZsGreen-attP | Bxb1-responsive fluorescent reporter plasmid containing reverse complementary ZsGreen flanked by Bxb1 attB/P recognition sites in head-to-head orientation |
| 51266 | pCAG-b3STOPb3-ZsGreen | B3-responsive fluorescent reporter plasmid containing a STOP-cassette flanked by B3-recognition sites followed by ZsGreen CDS |
| 51267 | pCAG-Co-InCreN | The N-terminal component of the Co-InCre system, expresses the N-Cre-N-gp41-1 fusion that generates functional Cre by protein trans-splicing upon reacting with Co-InCreC |
| 51268 | pCAG-Co-InCreC | The C-terminal component of the Co-InCre system, expresses the C-gp41-1-C-Cre fusion that generates functional Cre by protein trans-splicing upon reacting with Co-InCreN |
| 51269 | pCAG-loxPSTOPloxP-ZsGreen | Cre-responsive fluorescent reporter plasmid containing a STOP-cassette flanked by loxP sites followed by ZsGreen |
| 51270 | pCAG-NLS-HA-B3 | Expression of the site-specific recombinase B3 (mouse codon-optimized) |
| 51271 | pCAG-NLS-HA-Bxb1 | Component of the Bxb1-rcCre binary site-specific recombinase system, expression of the site-specific recombinase Bxb1 (mouse codon-optimized) |
| 51272 | pCAG-NLS-HA-Dre | Component of the Co-Driver binary site-specific recombinase system, expression of the site-specific recombinase Dre (mouse codon-optimized) |
| 51273 | pCAG-Roxed-Cre | Component of the Co-Driver binary site-specific recombinase system, generates functional rox60-71Cre upon removal of an internal STOP cassette by Dre |
| 51274 | pCAG-roxSTOProx-ZsGreen | Dre-responsive fluorescent reporter plasmid containing a STOP-cassette flanked by Dre-recognition sites (rox) followed by ZsGreen |
| 51275 | Thy1.2-NLS_HA_Dre-P2A-BFP | Co-expression of the site-specific recombinase Dre and BFP under the control of the Thy1.2 expression cassette |
| 51276 | Thy1.2-Roxed-Cre | Expression of the Dre-dependent Roxed-Cre gene under the control of the Thy1.2 expression cassette |
| 51277 | hGFAP-NLS-HA-Dre-P2A-BFP | Co-expression of the site-specific recombinase Dre and BFP under the control of the hGFAP promoter |
| 51278 | pCAG-Golden-Gate-Esp3I-Destination | CAG promoter-driven expression vector suitable for ligation of multiple inserts by Golden Gate Cloning using Esp3I |