Precise manipulation of bacterial chromosomes by conjugative assembly genome engineering.

Ma NJ, Moonan DW, Isaacs FJ
Nat Protoc. 2014 Oct;9(10):2285-300. doi: 10.1038/nprot.2014.081. Epub 2014 Sep 4. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID	Plasmid	Purpose
51946	rEc2	oriT-kan template
51948	rEc 27.H.Sc	specR template
51950	pRK24	broad-host range natural conjugative plasmid