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Cas9 effector-mediated regulation of transcription and differentiation in human pluripotent stem cells.
Kearns NA, Genga RM, Enuameh MS, Garber M, Wolfe SA, Maehr R
Development. 2014 Jan;141(1):219-23. doi: 10.1242/dev.103341.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
50915pHAGE TRE dCas9Tet-regulatable dCas9 lentiviral expression vector
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50916pHAGE TRE dCas9-VP64Tet-regulatable dCas9-VP64 2nd generation lentiviral expression vector
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50917pHAGE TRE dCas9-KRABTet-regulatable dCas9-KRAB 2nd generation lentiviral expression vector
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50918pHAGE EF1α dCas9-VP64Constitutive dCas9-VP64 lentiviral expression vector
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50919pHAGE EF1α dCas9-KRABConstitutive dCas9-KRAB 2nd generation lentiviral expression vector
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50920pLKO.1-puro U6 sgRNA BfuAI stufferU6 driven sgRNA cloning vector where guide sequences are inserted between BfuAI sites
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50921pLKO.1-puro U6 sgRNA Oct4A -12U6 driven sgRNA targeting OCT4 isoform A -12 bp from TSS
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50922pLKO.1-puro U6 sgRNA Oct4A -158U6 driven sgRNA targeting OCT4 isoform A -158 bp from TSS
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50923pLKO.1-puro U6 sgRNA SOX17 -91U6 driven sgRNA targeting Sox17 -91 bp from TSS
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50924pLKO.1-puro U6 sgRNA SOX17 -126U6 driven sgRNA targeting Sox17 -126 bp from TSS
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50925pLKO.1-puro U6 sgRNA SOX17 -177U6 driven sgRNA targeting Sox17 -177 bp from TSS
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50926pLKO.1-puro U6 sgRNA SOX17 -296U6 driven sgRNA targeting Sox17 -296 bp from TSS
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50927pLKO.1-puro U6 sgRNA CAGU6 driven sgRNA negative control
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