Cas9 effector-mediated regulation of transcription and differentiation in human pluripotent stem cells.
Kearns NA, Genga RM, Enuameh MS, Garber M, Wolfe SA, Maehr R
Development. 2014 Jan;141(1):219-23. doi: 10.1242/dev.103341. PubMed Article
Kearns NA, Genga RM, Enuameh MS, Garber M, Wolfe SA, Maehr R
Development. 2014 Jan;141(1):219-23. doi: 10.1242/dev.103341. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 50915 | pHAGE TRE dCas9 | Tet-regulatable dCas9 lentiviral expression vector |
| 50916 | pHAGE TRE dCas9-VP64 | Tet-regulatable dCas9-VP64 2nd generation lentiviral expression vector |
| 50917 | pHAGE TRE dCas9-KRAB | Tet-regulatable dCas9-KRAB 2nd generation lentiviral expression vector |
| 50918 | pHAGE EF1α dCas9-VP64 | Constitutive dCas9-VP64 lentiviral expression vector |
| 50919 | pHAGE EF1α dCas9-KRAB | Constitutive dCas9-KRAB 2nd generation lentiviral expression vector |
| 50920 | pLKO.1-puro U6 sgRNA BfuAI stuffer | U6 driven sgRNA cloning vector where guide sequences are inserted between BfuAI sites |
| 50921 | pLKO.1-puro U6 sgRNA Oct4A -12 | U6 driven sgRNA targeting OCT4 isoform A -12 bp from TSS |
| 50922 | pLKO.1-puro U6 sgRNA Oct4A -158 | U6 driven sgRNA targeting OCT4 isoform A -158 bp from TSS |
| 50923 | pLKO.1-puro U6 sgRNA SOX17 -91 | U6 driven sgRNA targeting Sox17 -91 bp from TSS |
| 50924 | pLKO.1-puro U6 sgRNA SOX17 -126 | U6 driven sgRNA targeting Sox17 -126 bp from TSS |
| 50925 | pLKO.1-puro U6 sgRNA SOX17 -177 | U6 driven sgRNA targeting Sox17 -177 bp from TSS |
| 50926 | pLKO.1-puro U6 sgRNA SOX17 -296 | U6 driven sgRNA targeting Sox17 -296 bp from TSS |
| 50927 | pLKO.1-puro U6 sgRNA CAG | U6 driven sgRNA negative control |