pHAGE EF1α dCas9-VP64
PurposeConstitutive dCas9-VP64 lentiviral expression vector
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||50918||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Total vector size (bp) 12806
Vector typeMammalian Expression, Lentiviral, CRISPR
Growth in Bacteria
Growth Strain(s)NEB Stable
Copy numberHigh Copy
Insert Size (bp)4406
- Promoter EF1alpha
/ Fusion Proteins
- 3xHA (C terminal on insert)
- VP64 (C terminal on insert)
- Cloning method Gateway Cloning
- 5′ sequencing primer AGAGCTCGTTTAGTGAACCG
- 3′ sequencing primer MSCV-rev (Common Sequencing Primers)
Please note that there are several mismatches (minor deletions and insertions) between depositor's reference sequence and Addgene's quality control sequence. The mismatches are in cloning junctions/non-coding regions and should not affect plasmid function.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pHAGE EF1α dCas9-VP64 was a gift from Rene Maehr & Scot Wolfe (Addgene plasmid # 50918 ; http://n2t.net/addgene:50918 ; RRID:Addgene_50918)
For your References section:Cas9 effector-mediated regulation of transcription and differentiation in human pluripotent stem cells. Kearns NA, Genga RM, Enuameh MS, Garber M, Wolfe SA, Maehr R. Development. 2014 Jan;141(1):219-23. doi: 10.1242/dev.103341. 10.1242/dev.103341 PubMed 24346702