pLKO.1-puro U6 sgRNA BfuAI stuffer
Purpose(Empty Backbone) U6 driven sgRNA cloning vector where guide sequences are inserted between BfuAI sites
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||50920||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
- Backbone size (bp) 7145
Vector typeMammalian Expression, Lentiviral, CRISPR
- Promoter U6
Growth in Bacteria
Growth Strain(s)NEB Stable
Copy numberHigh Copy
- Cloning method Restriction Enzyme
- 5′ sequencing primer CGTGACGTAGAAAGTAATAATTTC
- 3′ sequencing primer CCTCGAGCCGCGGCCAAAG (Common Sequencing Primers)
Plasmid contains an IS1 prokaryotic transposable element that does not affect plasmid function.
Please note that for improved cassette cloning efficiency the wolf lab recommends plasmid 52628.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pLKO.1-puro U6 sgRNA BfuAI stuffer was a gift from Rene Maehr & Scot Wolfe (Addgene plasmid # 50920 ; http://n2t.net/addgene:50920 ; RRID:Addgene_50920)
For your References section:Cas9 effector-mediated regulation of transcription and differentiation in human pluripotent stem cells. Kearns NA, Genga RM, Enuameh MS, Garber M, Wolfe SA, Maehr R. Development. 2014 Jan;141(1):219-23. doi: 10.1242/dev.103341. 10.1242/dev.103341 PubMed 24346702