Allen Institute for Cell Science Plasmid Collection
Background
The Allen Institute for Cell Science has produced the first publicly available collection of fluorescently tagged, human, induced pluripotent stem cell (hiPSC) lines. The plasmids used to generate these lines have been deposited here with Addgene for distribution to the research community.
With these plasmids researchers will be able to generate fluorescently tagged proteins of key cellular structures in human cell lines of their choice. For a full description of how to use these plasmids, check out Plasmids for Endogenous Gene Tagging in Human Cells on the Addgene Blog.
Allen Institute for Cell Science Plasmids
Find additional cell line information from the Allen Institute for Cell Science data portal:
- View data and images from the hiPS cell lines
- Find gRNA sequences used in the creation of the hiPS cell lines
The following plasmids can be used to create cell lines with endogeneously tagged cellular structures.
| Addgene ID | Plasmid | Allen Institute ID | Tag | Protein | Structure |
|---|---|---|---|---|---|
| 87420 | PXN-EGFP | AICSDP-1 | EGFP | Paxillin | Matrix Adhesions |
| 87421 | TUBA1B-mEGFP | AICSDP-4 | mEGFP | Alpha-tubulin | Microtubules |
| 87422 | LMNB1-mEGFP | AICSDP-10 | mEGFP | Nuclear laminB1 | Nuclear envelope |
| 87423 | TOMM20-mEGFP | AICSDP-8 | mEGFP | TOM20 | Mitochondria |
| 87424 | DSP-mEGFP | AICSDP-9 | mEGFP | Desmoplakin | Desmosomes |
| 87425 | ACTB-mEGFP | AICSDP-15 | mEGFP | Beta-actin | Actin |
| 87426 | SEC61B-mEGFP | AICSDP-7 | mEGFP | Sec61-beta | Endoplasmic Reticulum |
| 87427 | FBL-mEGFP | AICSDP-13 | mEGFP | Fibrillarin | Nucleolus |
| 87428 | MYH10-mEGFP | AICSDP-24 | mEGFP | Non-muscle myosin heavy chain IIB | Actomyosin bundles |
| 87429 | TJP1-mEGFP | AICSDP-23 | mEGFP | Tight junction protein ZO1 | Tight junctions |
| 91565 | AAVS1-mEGFP | AICSDP-35 | mEGFP | NA | Cytoplasm |
| 101781 | CETN2-mTagRFP-T | AICSDP-22 | mTagRFP-T | Centrin-2 | Centrosome |
| 101783 | MAP1LC3B-mEGFP | AICSDP-25 | mEGFP | LC3 | Autophagosomes |
| 101784 | SLC25A17-mEGFP | AICSDP-27 | mEGFP | Peroxisomal membrane protein PMP34 | Peroxisomes |
| 101785 | TUBA1B-mTagRFP-T | AICSDP-28 | mTagRFP-T | Alpha tubulin | Microtubules |
| 101786 | ST6GAL1-mEGFP | AICSDP-26 | mEGFP | Sialyltransferase 1 | Golgi |
| 109122 | NPM1-mEGFP | AICSDP-50 | mEGFP | Nucleophosmin | Nucleolus |
| 109120 | GJA1-mEGFP | AICSDP-43 | mEGFP | Connexin-43 | Gap junction |
| 109121 | HIST1H2BJ-mEGFP | AICSDP-52 | mEGFP | Histone H2B type 1-J | Nucleus |
| 109119 | CTNNB1-mEGFP | AICSDP-47 | mEGFP | Beta catenin | Adherens junctions |
| 107579 | RAB5A-mEGFP | AICSDP-39 | mEGFP | Ras-related protein Rab-5A | Endosomes |
| 107580 | AAVS1-mTagRFPT-CAAX | AICSDP-42 | mTagRFPT-CAAX | NA | Plasma Membrane |
| 114403 | LMNB1-mTagRFP-T | AICSDP-29 | mTagRFPT | Lamin B1 | Nuclear envelope |
| 114404 | AAVS1-mEGFP (PGK) | AICSDP-36 | mEGFP | NA | Cytoplasm |
| 114405 | ATP2A2-mEGFP | AICSDP-41 | mEGFP | SERCA2 | Sarcoplasmic Reticulum/Endoplasmic Reticulum |
| 114406 | SMC1A-mEGFP | AICSDP-57 | mEGFP | SMC protein 1A | Cohesin |
| 114407 | NUP153-mEGFP | AICSDP-50 | mEGFP | Nucleoporin Nup153 | Nuclear pores |
| 114409 | CBX1-mEGFP | AICSDP-59 | mEGFP | Chromobox protein homolog 1 | Heterochromatin |
| 114410 | FUS-mEGFP | AICSDP-64 | mEGFP | RNA-binding protein FUS | Paraspeckles/Stress Granules |
| 114411 | TNNI1-mEGFP | AICSDP-34 | mEGFP | Troponin I, slow skeletal muscle | Sarcomeric thin filament |
| 114412 | TTN-mEGFP | AICSDP-45 | mEGFP | Titin | Sarcomere (M-line tag) |
| 114413 | MYL7-mEGFP | AICSDP-46 | mEGFP | MLC-2a | Sarcomeric thick filaments |
IPSC cell lines can be ordered from the Allen Institute for Cell Science Cell Catalog.
Protocols
The Allen Institute for Cell Science has several protocol videos available that may be helpful for working with this plasmid collection.
Reference
Roberts B, et al. 2017. Systematic gene tagging using CRISPR/Cas9 in human stem cells to illuminate cell organization. Mol Biol Cell. 28(21):2854-2874. PMID: 28814507