RESOLUTE Plasmid Collection

Solute carrier proteins (SLCs) are the largest family of transporters in the human genome with approximately 450 identified members. Many SLCs have mutations associated with disease and could become targets for drug development. RESOLUTE is a public-private partnership of 13 members from industry and academia financed by the Innovative Medicine Initiative (IMI) of the European Union. The RESOLUTE mission is to develop data and reagents to boost the research focused on SLCs.

Through the coupling of an inclusive, ‘open-access ethos’ to the results, techniques, and reagents with the highest-possible quality of research output, RESOLUTE expects to accelerate the pace of research in the field of SLCs to the global benefit of basic academic research as well as applied research in small biotech and pharmaceutical companies.

The RESOLUTE project has received funding from the Innovative Medicines Initiative 2 Joint Undertaking under grant agreement No 777372. This Joint Undertaking receives support from the European Union's Horizon 2020 research and innovation programme and EFPIA.

Figure 1: Representation of RESOLUTE mission. The combination of metabolomics and genome engineering forms the core of the deorphanizing engine. Genetic and proteomic interactions represent the framework that provides biological context. Assays monitoring impedance, fluorescence, mass/charge properties, protein thermal stabilization, and changes of voltage all contribute to assess ligand action/engagement. Protein production is used for assays and to generate antibody/ high-affinity ligands. Data is embedded in an integrated knowledgebase that features, among others, genetic variants and disease associations.

Plasmids

This plasmid collection contains codon-optimized ORF sequences for human SLCs and SLC binder sequences in Gateway-compatible cloning vectors (pDONR, pENTR), bacterial expression vectors (pET22b, pBXNPFLAGH), and lentiviral vectors (pCW57.1). The table also includes empty vectors developed by the RESOLUTE Consortium.

Pooled Libraries

CRISPR and overexpression pooled libraries are useful tools for genetic screening experiments. These libraries target the solute carrier family of proteins.

Library	ID	PI	Description
Human SLC Activation Library	132561	Superti-Furga	CRISPR activation library targeting human SLC proteins.
Human SLC Knockout Library	132552	Superti-Furga	CRISPR knockout library targeting human SLC proteins.
Mouse SLC Knockout Library	141436	Superti-Furga	CRISPR knockout library targeting mouse SLC proteins.
Human SLC Overexpression Library	213694	Superti-Furga	Inducible barcoded overexpression library of human SLC proteins.
Human Transporter Knockout Libraries	213695 221409 221410	Superti-Furga	CRISPR knockout library targeting human transmembrane transporters.

Feedback

Please send your feedback to the Resolute Partnership using their RESOLUTE repository feedback form . If you have further questions or comments you can also contact them at .

Resources and References

Other Addgene Resources

• Brain Initiative Plasmid Collection
• NeuroMab Plasmid Collection
• Addgene Blog: Gateway Cloning

References

Frommelt, F., Ladurner, R., Goldmann, U., Wolf, G., Ingles-Prieto, A., Lineiro-Retes, E., Gelová, Z., Hopp, A. K., Christodoulaki, E., Teoh, S. T., Leippe, P., Santini, B. L., Rebsamen, M., Lindinger, S., Serrano, I., Onstein, S., Klimek, C., Barbosa, B., Pantielieieva, A., Dvorak, V., Hannich, T. J., Schoenbett, J., Sansig, G., Mocking, T. A. M., Ooms, J. F., IJzerman, A. P., Heitman, L. H., Sykacek, P., Reinhardt, J., Müller, A. C., Wiedmer, T., Superti-Fuga, G. (2025). The solute carrier superfamily interactome.. Mol Syst Biol, 21(6), 632-675. https://doi.org/10.1038/s44320-025-00109-1 PMID: 40355756

Goldmann, U., Wiedmer, T., Garofoli, A., Sedlyarov, V., Bichler, M., Haladik, B., Wolf, G., Christodoulaki, E., Ingles-Prieto, A., Ferrada, E., Frommelt, F., Teoh, S. T., Leippe, P., Onea, G., Pfeifer, M., Kohlbrenner, M., Chang, L., Selzer, P., Reinhardt, J., Digles, D., Ecker, G. F., Osthushenrich, T., MacNamara, A., Malarstig, A., Hepworth, D., Superti-Fuga, G. (2025). Data- and knowledge-derived functional landscape of human solute carriers.. Mol Syst Biol, 21(6), 599-631. https://doi.org/10.1038/s44320-025-00108-2 PMID: 40355757

Superti-Furga, G., Lackner, D., Wiedmer, T., Ingles-Prieto, A., Barbosa, B., Girardi, E., Goldmann, U., Gürtl, B., Klavins, K., Klimek, C., Lindinger, S., Liñeiro-Retes, E., Müller, A. C., Onstein, S., Redinger, G., Reil, D., Sedlyarov, V., Wolf, G., Crawford, M., Everley, R., Hepworth, D., Liu, S., Noell, S., Piotrowski, M., Stanton, R., Zhang, H., Corallino, S., Faedo, A., Insidioso, M., Maresca, G., Redaelli, L., Sassone, F., Scarabottolo, L., Stucchi, M., Tarroni, P., Tremolada, S., Batoulis, H., Becker, A., Bender, E., Chang, Y. N., Ehrmann, A., Müller-Fahrnow, A., Pütter, V., Zindel, D., Hamilton, B., Lenter, M., Santacruz, D., Viollet, C., Whitehurst, C., Johnsson, K., Leippe, P., Baumgarten, B., Chang, L., Ibig, Y., Pfeifer, M., Reinhardt, J., Schönbett, J., Selzer, P., Seuwen, K., Bettembourg, C., Biton, B., Czech, J., de Foucauld, H., Didier, M., Licher, T., Mikol, V., Pommereau, A., Puech, F., Yaligara, V., Edwards, A., Bongers, B. J., Heitman, L. H., IJzerman, A. P., Sijben, H. J., van Westen, G. J. P., Grixti, J., Kell, D. B., Mughal, F., Swainston, N., Wright-Muelas, M., Bohstedt, T., Burgess-Brown, N., Carpenter, L., Dürr, K., Hansen, J., Scacioc, A., Banci, G., Colas, C., Digles, D., Ecker, G., Füzi, B., Gamsjäger, V., Grandits, M., Martini, R., Troger, F., Altermatt, P., Doucerain, C., Dürrenberger, F., Manolova, V., Steck, A. L., Sundström, H., Wilhelm, M., Steppan, C. M. (2020) The RESOLUTE consortium: unlocking SLC transporters for drug discovery.. Nat Rev Drug Discov, 19(7):429-430. https://doi.org/10.1038/d41573-020-00056-6 PMID: 32265506

Wiedmer, T., Teoh, S.T., Christodoulaki, E., Wolf, G., Tian, C., Sedlyarov, V., Jarret, A., Leippe, P., Frommelt, F., Ingles-Prieto, A., Lindinger, S., Barbosa, B. M. G., Onstein, S., Klimek, C., Garcia, J., Serrano, I., Reil, D., Santacruz, D., Piotrowski, M., Noell, S., Bueschl, C., Li, H., Chi, G., Mereiter, S., Oliveira, T., Penninger, J. M., Sauer, D. B., Steppan, C. M., Viollet, C., Klavins, K., Hannich, J. T., Goldamn, U., Superti-Furga, G. (2025). Metabolic mapping of the human solute carrier superfamily.. Mol Syst Biol, 21(6), 560-598. https://doi.org/10.1038/s44320-025-00106-4 PMID: 40355754

Wolf, G., Leippe, P., Onstein, S., Goldamnn, U., Frommelt, F., Teoh, S. T., Girardi, E., Wiedmer, T., Superti-Furga, G. (2025). The genetic interaction map of the human solute carrier superfamily.. Mol Syst Biol, 21(6), 531-559. https://doi.org/10.1038/s44320-025-00105-5 PMID: 40355755

Credits

Contributing Authors

Written by researchers in the Superti-Furga group.
Reviewed by the Scientific Curation team at Addgene.

Last Updated

Content last reviewed on 30 January 2025. Catalog items are updated more frequently.