Fluorescent Proteins: Plasmids and Resources

Addgene's plasmid repository contains thousands of fluorescent proteins. Use this guide to learn more about the many applications of fluorescent proteins and to find plasmids for your next experiment.

Browse Fluorescent Protein Plasmids by Category

Empty Backbones

Understand the localization and/or function of your protein of interest by fusing it to a fluorescent protein.

Michael Davidson Fluorescent Protein Collection

Browse this extensive collection of ORFs tagged with a variety of fluorescent proteins as well as hundreds of empty backbones.

FRET

Find FRET pairs and standards to study protein-protein interactions or conformational changes within a protein.

Biosensors

Monitor small biomolecules or other physiological intracellular processes with genetically encoded fluorescent biosensors.

Subcellular Localization

Visualize organelles and subcellular structures with a well-characterized fluorescent fusion protein marker.

In Vivo Imaging

Image with far-red or infrared tools to study molecular processes in organs and whole mammals.

Additional Resources at Addgene

Optogenetics Guide | Optogenetics Plasmids

Use light to activate, inhibit, and control molecular pathways or cells.

Chemogenetics Guide | Chemogenetics Plasmids

Use small molecules to activate or inhibit engineered receptors that affect cell signalling pathways.

• Addgene Blog: Fluorescent Proteins
• Luciferase Plasmid Collection
• Fluorescent Protein Kits
• Allen Institute for Cell Science Plasmid Collection
• New England BioLabs Cell-Imaging Plasmid Collection
• Promega Plasmid Collection
• Botman-Teusink Yeast Fluorescent Protein Collection

External Resources

• FPbase : an open-source, community-editable database for fluorescent proteins created by Talley Lambert.
• Nikon MicroscopyU
• Olympus Microscopy Resource Center
• Leica Microsystems Resources for Microscopy in Life Sciences